目的:建立体外培养兔角膜缘上皮细胞的方法,观察其体外生长的生物学特性。
Objective:To establish a method for culturing limbal corneal epithelial cells of rabbit and to observe the growth characteristics of the cultured cells in vitro.
目的:探讨各种生长因子及其受体在正常角膜和角膜缘的表达及分布规律。
Purpose: To investigate the expression and distribution characteristics of growth factors and their receptors in normal cornea and limbus .
方法应用消化培养法对人胎儿角膜缘上皮组织进行体外培养并观察记录培养细胞的生长特性;
Methods Human fetal limbal epithelial tissue was cultured in vitro by enzymatic digestion culture method and the morphological characteristics of cultured cells were recorded.
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