挑战：关于实物捐赠，有一些特别的限制需要遵守，这很重要。

Challenges: There are special constraints associated with in-kind giving that are important to honour.

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这种硅碳棒在安装位置上没有什么特别的限制，通常采用立式的和卧式的。

There are no restrictions on the mounting positions of Elements, although the horizontal and vertical positions are common.

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本文试图给出一个普适的统一的理论公式，它考虑了空间电荷限制效应和温度的影响，并且对场强也没有特别的限制，可用于金属FEA的数值计算。

The paper give a generalized theory equation with space charge effect. It does not restrict the range of Temperature and Field strength, It can use for metal FEA numerical calculation.

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They do that in a very special way in that they - restriction enzymes are able to identify a particular sequence of bases in a gene.

限制性内切酶的工作机制很特别,它能识别基因中的一段特定的碱基序列

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That cutting is done by special proteins called restriction enzymes.

切割是由一种特别的蛋白质完成的,称为限制性内切酶

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Now if I put them in contact with one another, the plasmid that's been opened and fragments of the DNA - special fragments that I've produced with the same restriction enzyme, they'll have the same sticky ends, they will naturally hybridize with one another.

如果我让它们互相接触,已经切开的质粒和DNA片段--,这DNA片段是我已用同种限制性内切酶,处理过的特别片段,这两者会有相同的粘性末端,它们会自然地相互杂交

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