简介：以我区玉米育种所用骨干自交系花药或幼胚愈伤组织为受体，与携带着海藻糖合成酶基因TPS1和潮霉素抗性(Hygr)基因的双元载体质粒PCAMBIA1301的根癌农杆菌LBA4404共培养，将具抗性的胚性愈伤组织诱导成苗对其进行PCR鉴定。
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潮霉素抗性浓度的筛选为检测谷子转几丁质酶基因奠定基础。
The resistance concentration of hygromycin selected is the basis for testing transgenes millet plant.
幼胚诱导的愈伤组织，经潮霉素抗性筛选，得到可育的再生植株。
The regenerated fertile plants were obtained by effective selection for hygromycin B resistance.
将反义蜡质基因和潮霉素抗性基因通过共转化方法导入了籼稻9311，并进行了初步检测。
The antisense Waxy gene and hygromycin resistant gene were co-transformed into Indica rice 9311 and passed the basic detection.
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