重组菌经培养，利用荧光显微镜检测重组酵母的荧光强度，然后通过荧光分光光度计检测分选酶与底物相互作用后产生游离的EGFP荧光强度进而确定分选酶的酶活。

After cultivation, recombinant cells were verified with fluorescence microscopy and sortase activity was detected by fluorescence spectrophotometer from variety of free EGFP's fluorescence intensity.

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只要你的酵母中有活细菌，那么用这个基本酸奶制作方法你可以做出各种任何一种培养乳制品的不同版本。

You can use the basic yogurt method to make versions of any cultured dairy product, provided your starter carries live bacteria.

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