...予相应药物,观察肿瘤生长曲线和移植瘤重量,计算肿瘤抑制率;免疫组化方法检测增殖细胞核抗原(proliferation cell nuclear antigen,PCNA)在肿瘤组织中的表达;光镜和电镜观察肿瘤组织病理变化.
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方法:应用免疫组化法检测乳腺癌及相应癌旁组织tlr4、增殖细胞核抗原(pcna)的表达,TUNEL法检测组织中细胞的凋亡。
Methods: the expression of TLR4 and PCNA in cancer tissues and adjacent tissues were assessed by immunohistochemical method and the apoptosis was detected by the TUNEL method.
方法:应用SP免疫组化法,检测P 21, P 27等细胞周期依赖性激酶抑制剂和增殖细胞核抗原(pcna)在角膜上皮不同部位的表达情况。
METHODS: the expressions of CKI, P27, P21 and proliferating cell nuclear antigen (PCNA) were tested in different regions of corneal epithelium by SP immunohistochemistry.
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