... 阿尔金山北段：northern Altyn 氨基酸的置换：replacement of amino acid 阿拉斯加北端：Arctic Alaska ...
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单一氨基酸的置换可以改变酶的热稳定性。
Thermostability of enzymes can be altered by single amino acid substitution.
这两个点变异，将导致两个氨基酸的置换(Y154H、E348K)。
These substitution results in changes in two amino acid residues (Y154H and E348K).
进化酶基因的DNA测序表明4 186含两个有义氨基酸置换：k16 7r和S374C，二者既不位于底物结合位点，也不位于酶的金属离子结合位点。
DNA sequence revealed that 4-186 contains two amino acid substitutions, K167R and S374C, both of which locate neither the substrate - binding sites nor the metal - binding sites of EAP.
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