...tosterone，T）水平，MTT法检测ConA刺激T淋巴细胞增殖反应，RT-PCR法检测外周血单个核细胞（Peripheral blood mononuclear cells，PBMCs）、T淋巴细胞和B淋巴细胞雌激素受体α（Estragen receptor，ERα）mRNA的表达。
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...巴细胞(peripheralbloodlymphocyte,PBL)与靶细胞SKOV3结合、MTT法检测外周血单个核细胞(peripheralbloodmononuclearcells,PBMCs)增殖及PBL对靶细胞SKOV3杀伤效应,ELISA法检测杀伤过程中PBL分泌hIFN-γ、hTNF-..
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目的：探讨血液透析患者血清和外周血单个核细胞(PBMC)中输血传播病毒(TTV)检测状况及意义。
Objective: To study the situation and significance of transfusion transmitted virus (TTV) in serum and peripheral blood mononuclear cell (PBMC) of hemodialysis patients.
目的检测白细胞介素-2（IL-2）联合IL-12诱导人外周血单个核细胞（PBMC）体外抗肿瘤作用。
Objective To examine the in vitro antitumor activity of normal human peripheral blood mono-nuclear cells(PBMC) induced by interleukin-2(IL-2) and IL-12.
方法采用RT P CR技术、硫化pcr结合限制性内切酶技术检测白血病细胞系及正常人外周血单个核细胞WT 1基因的表达及其启动子区DNA甲基化水平。
Method The expression of WT1 gene and its DNA methylation status were assayed in leukemia cell lines and normal peripheral blood mononuclear cells (PBMNC) by RT-PCR and MS-PCR.
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