是一种编码可被检测的蛋白质或酶的基因,类似于报告基因,已经应用于植物基因工程和动物基因工程的研究领域。在植物基因表达调控的研究中,如花色苷代谢中,如查尔酮CHS基因,转录因子MYB10基因等。
摘要13 关键词: 胰岛素;前体脂肪细胞;增殖;分化;标志基因[gap=1323]Key words: insulin; preadipocyte; proliferation; differentiation; marker gene
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Results Microinjection of zili-MO,which knocked down the expression of zili,downregulated the expression of the ectodermal and mesodermal marker gene gsc,promoting the expression of the ectodermal marker gene eve1 and resulting in the decrease of endodermal cell expressed sox17.
结果整胚原位杂交检测结果表明,在注射了zili-MO后zili表达下调,外胚层及中胚层标志基因gsc的表达减弱,外胚层标志基因eve1的表达增强,表达sox17基因的内胚层细胞数量减少。
参考来源 - zili在斑马鱼早期胚胎发育胚层分化中作用的初步研究·2,447,543篇论文数据,部分数据来源于NoteExpress
配型涉及10组蛋白,或者标志基因;越多标志基因可以成列,则表示配型越是接近。
There are 10 different proteins, or markers, involved; the more markers that line up, the closer the match.
成骨细胞、软骨细胞以及脂肪细胞特异相关或标志基因的表达用RT - P CR检测。
Expression of osteoblast specific genes, chondrocyte specific genes, and adipocyte specific genes were confirmed by RT-PCR.
研究结果表明,该基因可用于多种肿瘤的诊断标志基因,也可以作为肿瘤基因治疗的靶基因。
The research result shows that: it can be applied to diagnostic sign gene for multiple tumours, and also be used as the target gene for the tumour gene therapy.
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