...是将细胞和培养基一次性加入反应器内进行培养,此后细胞不断增长,产物不断形成和积累,最后将条件培养基( conditioned medium),即经培养已含有细胞产物的培养基,或连同细胞一并取出,培养结束。
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当PHA白细胞条件培养基(PHA-LCM)被添加到培养的单核细胞/巨噬细胞分化的青睐。线(N-甲基乙酰胺,阿糖胞苷,或视黄酸)的HL-60细胞的分化诱导剂并无一致的效果上...
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淋巴细胞条件培养基 lymphocyte conditioned medium ; LCM
细胞条件培养基 conditioned medium ; Sertoli preconditioned medium ; Embryonic stem cell conditioned medium ; A549CM
单核细胞条件培养基 monocyte conditioned media ; MCM ; mononuclear cell conditioned medium ; mnc-cm
白细胞条件培养基 leucocyte-conditioned medium
的条件培养基 condition medium
BRL条件培养基 BRL conditioned medium
的内皮细胞条件培养基 endothelial cell conditioned media
小胶质细胞条件培养基 MGCM
脾细胞条件培养基 spleen cell-conditioned medium
The conditioned medium for low passage DPC can resume some properties of DPC.
低代毛乳头条件培养基可恢复毛乳头细胞部分生长特性。
参考来源 - 人毛乳头细胞在不同传代时期某些细胞因子表达的变化50% conditioned media prepared with cultured medium of adult limbal stem cell could not induce MSCs to transdifferentiate into corneal epithelial cells.
用成人角膜缘干细胞培养液制成的50%条件培养基对诱导人骨髓间充质干细胞横向分化为角膜上皮细胞无明显促进作用。
参考来源 - 体外诱导人骨髓间充质干细胞横向分化为角膜上皮细胞的可行性Culturing confluent BMSCs was induced along osteoblastic differentiation for 2 weeks in conditional medium,alkaline phosphatase was found positive expression in most of the cells. Calcium deposited on the extracellular matrix was detected after being induced for 3 weeks.
经过成骨条件培养基诱导分化后2周,细胞碱性磷酸酶染色阳性,3周后可以形成钙结节,证明所培养细胞可以向成骨细胞分化。
参考来源 - PLGA仿生矿化及其对大鼠BMSCs粘附增殖和成骨分化影响的实验研究The conditional media were collected from the primary cultures and subsequently analyzed using a liquid chromatography coupled with linear ion trap for the proteome.
随后采用液相色谱和线性离子阱质谱(Linear iontrap,LTQ)对条件培养基样品的总蛋白进行分离和全谱鉴定。
参考来源 - 人非小细胞肺癌相关游离蛋白的研究·2,447,543篇论文数据,部分数据来源于NoteExpress
结果:巨噬细胞条件培养基有促进脊髓腹侧神经元突起生长的作用。
Results: Macrophage conditioned media could promote the neurites outgrowth of the spinal ventral neuron.
方法取5个月龄水囊引产胚胎的下丘脑和垂体组织进行培养,并收集其条件培养基。
Methods the hypothalamic and pituitary tissues from freshly aborted 5-month human fetuses were taken for cellular culture and the condition medium was collected.
目的探索从骨肉瘤细胞条件培养基中提取骨形态蛋白(BMP)的方法,并测定其生物学活性。
Objective: To find out a method of extraction and purification of bone morphogenetic protein (BMP) from osteosarcoma cell conditioned medium, and evaluate the biological activity of BMP.
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