1）用金属螯合环氧载体EupergitC250L固定重组木聚糖酶XynB，得到固定化酶；2）将步骤1）得到的固定化酶悬浮于0．5－1．25M、pH为5．5－8．0磷酸盐缓冲液中，20－35℃反应12－60小时，得到固定化木聚糖酶；所述重组木聚糖酶XynB是将核苷酸序列是序列表中序列1的DNA序列的木聚糖酶基因经大肠杆菌表达得到的木聚糖酶。
本发明公开了一种制备木二糖的方法及其专用固定化酶。
This invention discloses a method for preparing xylobiose and its specific immobilization enzyme.
用TLC法测定研究所得的产物是以木糖、木二糖为主的低聚木糖产品。
The TLC results showed that the main components in the products were xylose and xylobiose.
结果表明，该方法重现性好，木二糖迁移时间和峰面积的相对标准偏差分别在0.5%和2.0%以内。
The result indicated that the relative standard deviations for xylobiose's migration time and peak area were all less than 0.5%, and 2.0% respectively.
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