一定时期的暗培养是翠冠叶片再生的必要条件。
Dark treatment was necessary for regeneration of Cuiguan leaves in the experiment.
暗培养有利于愈伤组织生长，对控制褐化也有一定作用。
Culturing in dark would benefit the growth of callus and control of brown stain.
添加激素i和暗培养有利于愈伤组织形成色素，诱导率达30%。
Adding of hormone I in dark culture, it was favourable to the pigments formation in callus. The inducement radio was 30%.
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