介绍了细菌荧光显微镜直接计数法的基本原理、仪器设备、分析和计算方法，并对水样过滤操作方法作了一些改进。
This article introduces the basic principle, instrument, analysis and calculative method on how to take count of bacteria by fluorescence microscope.
所采样品冷藏带回，应用荧光显微镜计数细菌总数，倒平板法计数弧菌总数。
Bring the sampling to the laboratory under low temperature circumstances, then, census the total numbers of bacteria by fluorescence microscope and vibrio by selection technique of plate cultivation.
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