染色体整合位点 chromosomal integration site
Previously, some transgenic detectin methods had been reported, but the detection method is simplistic not the general screening and identifying method. In abroad, the study of integration site used for transgenic detection had just begun.
有关转基因检测的方法,国内外已有不少报道,但具有单一性,没有通用的筛选、鉴定方法,有关整合位点的研究国外刚刚开始,国内未见报道。
参考来源 - 转基因植物外源基因的检测方法及整合位点的研究·2,447,543篇论文数据,部分数据来源于NoteExpress
前病毒整合位点、靶细胞特性和整合后的载体结构与转入基因表达的稳定性紧密相关。
The integration sites of provirus, the nature of the target cells and the structure of vectors were closely related to the stability of gene expression.
目的:为分析慢病毒介导的转基因小鼠中外源基因整合位点的信息,应用反向PCR克隆整合位点序列。
Objective: To investigate the information of transgene integrations in mice mediated with lentiviral vectors, the inverse PCR was used to clone the sequences of integration sites.
通过慢病毒整合位点鉴别,我们得以分辨转移和非转移瘤并明确可区分这两种不同类型肿瘤的基因表达改变。
Identification of the lentiviral integration sites allowed us to distinguish metastatic from non-metastatic tumours and determine the gene expression alterations that distinguish these tumour types.
The first step is we had to be able to take this circular DNA and cut it to create a site for our new gene to be added.
第一步是切割环形DNA,以创造出一个整合新基因的位点
We're going to have DNA that we would like to make lots of copies of, or we're going to have DNA that we would like to get expressed in a cell, and we're going to put it in this region that's called the polylinker.
我们将那些需要得到很多拷贝数的DNA,或者需要在细胞中表达的DNA,整合到这个叫做多克隆位点的区域中
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