摇瓶培养是在菌种的筛选培养阶段(中试),培养条件要尽可能和发酵生产的培养条件相近,但工作量大、时间长、操作复杂;而自动发酵罐培养是摇瓶培养的工业化生产。
... 渗析培养 dialysis culture 摇瓶培养 shake-flask culture 无血清培养 serum-free culture ...
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On the basis of shake-flask culture and fetch fermentation, the coupling of semi-continuous fermentation and ultrafiltration was developed on chitosan-oligosaccharide preparation in the 5 L fermentation tank.
在摇瓶培养和分批培养的基础上,在5L的发酵罐中,通过半连续发酵和超滤耦合制备不同分子量壳低聚糖。
参考来源 - 发酵和分离耦合制备壳低聚糖的研究Different cultivation condition such as induced time, methanol concentration and pH of culture medium was studied at shake flask cultivation.
通过摇瓶培养初步摸索了诱导时间、甲醇浓度、培养基pH值等培养条件对Mut~+重组菌株表达PAP的影响。
参考来源 - 美洲商陆抗病毒蛋白基因在毕赤酵母中的表达The optimum shaking culture condition was as follows: initial medium pH 6.5, 10% inoculation quantity, 160r/min shaking frequency, 100ml medium in 250ml shaking flask, 6 days incubation.
结果表明最佳的摇瓶培养条件为:起始pH值6.5、装液量80ml/250ml、接种量10%、摇床转速160rpm,发酵周期为6d。
参考来源 - 白灵菇液体发酵及其胞外多糖生物活性的研究·2,447,543篇论文数据,部分数据来源于NoteExpress
采用摇瓶培养法对发酵培养基配方进行了研究。
Formula of fermentation medium was studied by flask fermentation.
方法:富集培养、血平板分离、摇瓶培养和排油圈测定。
Method: Based on enrichment culture, blood plate separation, the flask culture and exclusive circle of methods such as oil.
摇瓶培养被广泛用于新抗生素菌种的筛选和选育以及培养条件的确定。
Shaking flask culture is being widely used for the evaluation of a new antibiotic producing strain in screening programme or to ascertain the culture condition of a certain microorganism.
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