然后在该培养基配方的基础上进行摇瓶分批补料发酵试验，确定最佳补料方案。
The fed-batch fermentation method was studied by flask culture based on the optimal medium.
本文研究了玉米皮水解液发酵生产饲料酵母的摇瓶间歇培养和分批补料培养条件，进行了10L反应器分批补料培养试验。
The biotechnology of flask batch and fed-batch culture to produce yeast from cornhusk hydrolysate was studied and the fed -batch process in 10L bioreactor was conducted.
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