结果 表明,小麦花药培养中的愈伤组织诱导效率与培养容器大小无关,与花药接种密度显著相关 ;
The result showed that the induction frequency of pollen callus was irrelevant to the volume of culture containers, and highly related to the density of pollens.
前言: 目的:建立人M5型白血病细胞系SHI-1/S CID(重症联合免疫缺陷)小鼠模型,探讨白血病细胞的接种密度与SCID小鼠成瘤率及病理变化的关系。
AIM: To establish a model of human M5 leukemia and investigate the pathomorphological change in severe combined immunodeficient (SCID) mice after being transplanted with SHI-1 cells.
方法:1、应用MTT方法在细胞水平上进行中药粗提物的筛选,首先建立人肾脏 近 端 小 管上皮细胞株(HK-2)及卵巢癌细胞株(OC-3)的生长曲线,以确定接种密度及给药时机;
Methods: 1. In vitro, with MTT, the growth curve of HK-2 cells and OC-3 cells had been built in order to decide the density to inoculate and when to administration.
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