因为这将会减少沾染的背景和增加信号谣传比，所以沾染抗体的一个滴定很重要做。
A titration of the staining antibody is important to do because this will decrease background staining and increase signal to noise ratio.
(L 858r特异突变)的兔源单克隆抗体(43b2)(生物素化)能检测内源l 858r位点突变的EGFR蛋白的水平。为获得最佳特异性本抗体需要仔细滴定。
EGFR (L858R mutant specific) (43b2) Rabbit mAb (Biotinylated) detects endogenous levels of EGFR mutant L858R protein. Careful titration of this antibody may be required to obtain optimal specificity.
方法采用方阵滴定法，比较4种封闭液、不同稀释度的捕获抗体、检测抗体和辣根过氧化酶标记链菌素对检测结果的影响，确定ELISA法的最佳实验条件。
Method 4 kinds of block buffer, the different concentration of anti-MIF and biotin-anti-MIF, the different dilution of streptavidin-HRP are used for titration of ELISA test.
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