方法：PCR循环测序法是将PCR扩增与核酸序列分析相结合的一种研究方法。
Methods: PCRbased cycle DNA sequencing is a method that combines PCR amplification with DNA sequencing.
通过对随机挑选的23个片段进行克隆与测序，结果发现23个片段都是对应引物的RAPD扩增产物。
It was found these 23 fragments, which were cloned and sequenced after randomly selected, were the products from their corresponding RAPD primer amplification.
方法采用聚合酶链反应（PCR）扩增12例JAK2V617F点突变阴性PV患者的JAK2外显子12片段，经基因测序与野生型JAK2外显子12比对，了解是否存在JAK2外显子12突变。
Methods Polymerase chain reaction(PCR)was used for 12 JAK2V617F -negative PV patients to amply the region of JAK2 exon 12, direct gene sequencing was performed to detect mutations of JAK2 exon 12.
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