方法分别以rSj26-Sj32融合蛋白和日本血吸虫成虫抗原（SjAWA）作为包被抗原,通过Immunogold-IgG-Dot-ELISA检测慢性日本血吸虫病患者血清抗体,同时以华支睾吸虫病、卫氏并殖吸虫病、泡型棘球蚴...
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将合成多肽分别与钥孔戚血蓝蛋白(KLH)连接后免疫昆明小鼠,第3次免疫后收集血清,检测其针对各个多肽和可溶性成虫抗原(AWA)的IgG抗体滴度,以及补体介导的小鼠体外杀日本血吸虫童虫作用.
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方法用SDS-PAGE分析蚯蚓可溶性抗原(PaAg)、血吸虫可溶性成虫抗原(SjAg)、并殖吸虫可溶性成虫抗原(PwAg)、囊尾蚴囊液抗原(TcAg)和旋毛虫可溶性幼虫抗原(TsAg)的蛋白组分;并将其蛋白组分分别与血吸虫病、并殖吸虫病、猪囊尾蚴病和旋毛虫...
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The recombination protein SjEno/HIS was successfully purified .2 The enolase protein was recognized by the rabbit sera against to adult worm antigen in western analysis . It suggested good antigenicity.
2经Western blotting显示表达产物能被日本血吸虫成虫抗原免疫兔血清所识别,具有良好的抗原性。
参考来源 - 日本血吸虫重组烯醇化酶研究·2,447,543篇论文数据，部分数据来源于NoteExpress
以血吸虫成虫抗原（SWAP）及虫卵抗原（SEA）作为诊断抗原获得的结果差异不明显。
The similar results were obtained by using SWAP, SEA as diagnosing antigen comparing with that of recombinant LHDSj23/pGEX.
研究了姜片吸虫成虫冷浸抗原检测姜片吸虫病患者血清抗体的敏感性和特异性及其在流行病和临床上的应用价值。
This paper studied sensitivity, specificity and practical value and significance of ELISA in the detection of antibodies against Fasciolopsis buski in human sera.
方法用SDS- PAGE和双向电泳方法，对日本血吸虫两性成虫全虫可溶性抗原进行分析。
Methods The soluble antigens of the parasites were analysed by means of SDS PAGE and two dimensional gel electrophoresis.
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