组织被随机分配到新鲜组、玻璃化冷冻组和慢速冷冻组。

The tissues were randomly allocated to fresh, vitrification and conventional slow freezing groups.

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以常规的慢速冷冻法为对照，研究了玻璃化冷冻法保存绵羊体外受精囊胚的效果。

Then, the PGCs were froze respectively in six kinds of freezing medias under slow speed cryopreservation process and vitrification process.

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结论慢速冷冻结合植冰是小鼠卵巢组织冷冻保存比较有效的方案，但是冷冻方案尚需进一步完善。

Conclusion: in mouse, slow cooling with seeding is an optimal protocol for ovary tissue cryopreservation, but there is still something left to improve the cryopreservation protocol.

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