如果不涉及到内嵌函数，这个作用域链是唯一引用到调用对象的。

When no nested functions are involved, the scope chain is the only reference to the call object.

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在塔上安装大吨位的斜拉索时首次采用两道索夹牵引的方法，减小了导链引索的引力，有效避免了斜拉索的滑脱。

A method of two cable-nip traction is first applied when large tonnage cables are installed, all these decrease draught force of leading chain, and avoid surging for stayed cable efficiently.

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You then denature again, each one of these strands gets separated, primers bind, turn on the polymerase, a new strand is made.

然后你可以再次使产物链变性,使各链解旋,再结合引物,激活聚合酶,再生成新链

耶鲁公开课 - 生物医学工程探索课程节选

You first separated your DNA, let the primers bind, then turn on the enzyme, and it makes copies of each one of these.

首先解旋DNA双链,使引物结合,接着激活聚合酶,最终得到各链的复制产物

耶鲁公开课 - 生物医学工程探索课程节选

When they separate, the primers that you've added automatically bind through the process of hybridization, and then you turn up the temperature to the optimum for Taq polymerase and DNA synthesis starts.

当双链解旋后,加入的引物就会在杂交的步骤中自动,和解旋后的DNA结合,然后你再将温度升高至,Taq酶的最适温度,DNA就开始合成了

耶鲁公开课 - 生物医学工程探索课程节选