...察 病杂志, 2005(3): 177-178 【关键词】骨髓基质干细胞 细胞移植 DAPI 【文 摘】目的建立骨髓基质干细胞(marrow stromal cell，MSC)内耳移植技术，研究内耳细胞移植的可行性。
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目的： 分离培养睾丸支持细胞、骨髓基质细胞、胚胎成纤维细胞和胰腺成纤维细胞，优化培养条件，建立饲养细胞系，为干细胞体外共培养体系的建立提空充足的饲养细胞。
Objective: Sepretae Sertoli cell, BMSC, MEF, Pancreatic fibroblast and establish cell lines, constructed different feeder layers for a source as a coculture system for stem cells.
目前已建立了体外培养骨髓基质干细胞的方法，而且正在摸索进一步纯化的方法和诱导分化的条件。
Cultivation, purification, as well as induction methods have been developed and that will definitely lead to further clinical application of the cells.
本实验主要利用骨髓基质干细胞的这一特性，对其进行成软骨和成骨诱导，建立诱导和鉴定体系；
This experiment mainly used this feature of bone marrow stromal stem cells to find ways to induce them into chondrocytes and osteoblasts, and also established the induction and appraisal system;
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