采用反相层析图谱和毛细管电泳验证活性成分达到一定纯度。
RP-HPLC and capillary electrophoresis showed that the active fraction had reached some purify.
样本用丙酮提取，加二氯甲烷萃取，通过中性氧化铝和活性炭混合柱层析净化，然后上气相色谱用NPD检测。
The procedure was described, including extraction by acetone and dichloromethane, cleaning up by passing through a column made up of neutral alumina and active carbon, determination by GC with NPD.
采用亲和层析和离子交换层析分离纯化，以细胞增殖实验测定表达蛋白的生物活性。
Affinity chromatography and ion exchange chromatography were applied in isolation and purification, and the bioactivity of expression protein was determined in cell proliferation test.
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