方法应用免疫组织化学方法检测erk在脊髓后角的定位表达,并用图像分析系统和免疫印迹法进行定量分析。
Methods The expression of ERK in spinal dorsal horn was detected by immunohistochemical method and was quantitated by western-blot analyses.
结论:在非肾脏上皮细胞获得了稳定质膜定位表达UT - A2的FRT细胞株,该细胞株可用于UT - A 2抑制剂的筛选。
CONCLUSION: We constructed an FRT cell line that could stably express UT-A2 in plasma membrane in the non-renal epithelia cell. The cell line will be used to screen UT-A2 inhibitors.
表2展示了定位元素的DOM表达式。
应用推荐