方法：应用免疫组化法检测乳腺癌及相应癌旁组织tlr4、增殖细胞核抗原(pcna)的表达，TUNEL法检测组织中细胞的凋亡。
Methods: the expression of TLR4 and PCNA in cancer tissues and adjacent tissues were assessed by immunohistochemical method and the apoptosis was detected by the TUNEL method.
正如MTT法和计数pcna阳性细胞比例所显示：瘦素的给予与鼠np细胞的增殖成剂量依赖性关系。
Leptin administration led to increased proliferation of rat NP cells in a dose-dependent manner, as shown by MTT assay and by counting the percentage of PCNA-positive cells.
MTT法显示转染组的细胞增殖力低于未转染组和转染空载体组（P<0.05）。
The MTT showed cell proliferation ability was lower in the transfection group than in the non-transfection and mock-vehicle groups (P<0.05).
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