The two full-length gene were 750 bps and 1250 bps in length,respectively,with coding regions of 515bps and 678 bps,encoding two proteins of 172 a. a. and 225 a. a,in the order.
这两个基因全长分别为750bp和1250bps,编码区长度为515bps和678bps,编码172个和225个氨基酸。
参考来源 - 花生种子全长cDNA文库的大规模测序和生物信息学分析·2,447,543篇论文数据，部分数据来源于NoteExpress
从中确定候选基因七个，利用电子延伸技术获得候选基因全长或5’、3’端。
Full-length or 3'and 5'sequences of seven genes were forecasted by electronic elongation.
结论：成功克隆SARS冠状病毒核衣壳蛋白基因全长，并在酿酒酵母中诱导表达成功，有助于抗sars分子疫苗的研究。
Conclusion Successful clone of an integrity SARS-CoV nucleocapsid gene and its effective expression in saccharomyces cerevisiae can be beneficial to the research on SARS vaccine.
目的：表达人免疫相关鸟苷三磷酸酶基因(IRGM) a全长融合蛋白，制备高质量的兔抗人IRGM多克隆抗体。
Aim: To express the whole length fusion protein of human IRGMa and prepare high quality rabbit anti-human immune-related gene guanosine triphosphate (IRGM) polyclonal antibody.
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