在丝状噬菌体pⅢ 基因一侧插入特定长度的随机寡核苷酸,从而在噬菌体表面展示与pⅢ融合的随机多肽。由于每一个噬菌体呈现一种序列的外源多肽,那么展示特定长度各种不同序列外源多肽的噬菌体集合体就构成一个完整的随机多肽展示库。
结论采用筛选噬菌体展示文库的方法成功获得抱雌沟蛋白相互作用分子。
Conclusion Interaction proteins or peptides of SjGCP are successfully obtained through screening the T7 phage display library.
目的进一步改造噬菌体展示载体,使之能更有效地展示各种外源随机多肽文库。
Objective to further reconstruct the new phage display vector for more effectively displaying the foreign random peptide library.
噬菌体展示技术是将随机多肽文库表达于丝状噬菌体的表面,筛选功能性多肽的一种生物技术。
Phage-displayed peptide library technology is based on expressing recombinant random peptides on the surface of filamentous phage and selecting function peptides.
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