慢病毒载体用于眼部基因转移的实验研究-眼科学-论文中国 的治疗提供前期研究基础。方法含绿色荧光蛋白(greenfluorescentprotein,GFP)和内皮抑素(Endostatin,ES)两种外源基因及巨细胞病毒(cytomegalovirus,CMV)和视紫红质(rhodopsin,R
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慢病毒载体用于眼部基因转移的实验研究-眼科学-论文中国 的治疗提供前期研究基础。方法含绿色荧光蛋白(greenfluorescentprotein,GFP)和内皮抑素(Endostatin,ES)两种外源基因及巨细胞病毒(cytomegalovirus,CMV)和视紫红质(rhodopsin,R
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和重组人内皮抑素 recombinant human endostatin ; RHES
目的探讨反义vegf基因和内皮抑素基因联合转染在抑制肿瘤血管生成和肿瘤生长转移中的作用。
Objective to explore the co operative inhibitory effect of antisense VEGF gene and endostatin gene transfection on tumor angiogenesis, tumor growth and metastasis of lung cancer.
方法应用酶联免疫吸附试验(ELISA法),测定44例原发性肺癌、20例肺部良性疾病和20例正常人血清内皮抑素含量。
Methods Serum levels of endostatin were measured by an enzyme-linked immunosorbent assay (ELISA) in 44 patients with lung cancer, 20 patients with benign lung diseases and 20 normal persons.
方法用PCR方法扩增il_3信号肽序列和人内皮抑素基因,然后采用重叠延伸pcr拼接的方法将两者拼接和扩增。
METHODS IL_3 signal sequence and human endostatin gene were amplified with PCR and spliced with overlap extension PCR.
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