...疫细胞化学等方法研究了grp75的表达变化，并构建了grp75的过表达和低表达的PC12细胞模型，用台盼蓝拒染法(typan blue exclusion)和MTT法检测了不同细胞模型在缺糖培养后的细胞活力，同时对不同PC12细胞模型的的ATP、ROS以及线粒体跨膜电位的变化进行检测。
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方法采用台盼蓝拒染法，在不同条件下测定细胞增殖的抑制率。
Methods The inhibitory rates of cell growth were tested by trypan blue stain assay in the different conditions.
采用台盼蓝拒染法检测ATRA对SY5Y细胞的体外抗增殖作用；
Trypan blue exclusion was used to detect the antiproliferative effect of ATRA on SY5Y cells.
还用台盼蓝拒染法确定细胞活力以及培养AML细胞离心涂片细胞化学染色观察细胞形态。
The viability of cultured AML cells was also evaluated by Trypan blue exclusion. Cytospin specimens of cultured AML cells were studied by cytochemical staining.
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