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目的:探讨激光扫描共聚焦显微镜和荧光双重标记技术对心肌连接蛋白43进行定量和定位研究的方法。
Objective: To explore a method of detecting the connexin 43 (Cx43) quantity and distribution by laser scanning confocal microscope (LSCM) with a double label technique.
方法免疫荧光组织化学双重染色,染色结果在激光共聚焦扫描显微镜下观察。
METHODS Immunofluorescence histochemical double staining technique was used and the staining results were observed with confocal laser scanning microscope.
结论胃低张双重造影和CT扫描密切结合,对提高胃平滑肌肉瘤的诊断和鉴别诊断很有帮助。
Conclusion Hypotonic double contrast examination combined with CT scan, it is very helpful to improve the diagnostic and differential ability of gastric leiomyosarcoma.
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