检测开始时、先用试剂盒提供的PKA双酰胺罗丹明110肽底物进行一个标准的激酶反应。
The assay begins with a standard kinase reaction performed in the provided reaction Buffer with a provided Bisamide Rhodamine 110 peptide substrate (PKA R110 substrate).
结果：该双抗体夹心法，底物显色反应清楚肉眼即可作出判定，非特异干扰极小。
Results: The colored reaction of sandwich assay is very clear and easily judged by naked eyes.
通过与模拟实验进行比较，认为水解产物乙酰乙酸与AA一起充当了振荡反应双底物的角色。
It is considered that the hydrolysis acid and AA are the double substrates in the oscillating reaction through the comparison of simulation test.
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