对每个肿瘤三个不同区域进行免疫组化和银原位杂交，以判定HER2基因扩增的区域异质性和遗传异质性。
Regional and genetic heterogeneity of HER2 gene amplification was determined in three different areas of each tumor by immunohistochemistry and silver in situ hybridization.
原位PCR技术的基本步骤包括标本处理、蛋白酶消化、原位PCR扩增和扩增后产物的检测。
The elementary procedure of this technology contains sample disposal, protein enzyme digestion, in-situ PCR and the detection of sequence copied.
方法MTT方法观察对照组、RA处理组细胞的增殖变化，原位杂交检测两组细胞中msx基因扩增水平的变化。
Methods To observe the proliferation of the cells by MTT and the expression of MSX gene in the cells between ra treated and control group.
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