单限制性酶切AFLP SAFLP
This study first applied the single endonuclease digestion cDNA-AFLP to analyzethe differentially expressed genes in maize, and made a series of improvement to thetechnology to find some differentially expressed fragments related to opposite character inmaize .
本研究首次将单酶切 cDNA-AFLP技术应用于玉米基因的差异表达研究,并对其加以改进,成功分离到玉米对生性状相关基因差异表达片段。
参考来源 - 利用单酶切cDNA·2,447,543篇论文数据，部分数据来源于NoteExpress
最后用限制性内切酶单酶切及琼脂凝胶电泳进行鉴定。
Finally, we could evaluate plasmids cDNA extracted with mono-restriction endonuclease enzyme and the AGAR gel electrophoresis.
建立和优化了大麦dna单限制性酶切选择性扩增多态性技术体系(SADF)。
A method named selective amplification DNA fragments (SADF) using single restrictive enzyme was established and optimized in barley.
TILLING技术的核心是使用单链核酸内切酶大规模筛选突变体库。
The core technology in TILLING is screening mutant library by using single-strand endonuclease.
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