蛋白质的分级分离 在凝胶层析中，样品中有很多组分，分子量有一定差异，但是又没有达到组别分离的要求，因此选用排阻限度略大于最高分子量组分的凝胶类型，在凝胶层析过程中这些组分都能不同程度地渗透到凝胶内部，由于分配系数Kd不同，最后在凝胶层析中可能得到分离。分级分离的目的在于使各组分的Kd差异更大。
... 积分分离、integral action separation 分级分离、fractional separation 分离式分离机、separating centrifuge ...
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It was then digested by the restriction endonuclease Sfi I. After fractionation the cDNA fragments were connected to plasmid vector and a mixed full-length cDNA library was constructed successfully in the peanut.
利用限制性内切酶Sfi I酶切。 将经过分级分离得到的cDNA片段连接到质粒载体pDNR-LIB,成功构建花生各组织混合全长cDNA文库。
参考来源 - 花生各组织混合全长cDNA文库的构建和RGA片段的克隆·2,447,543篇论文数据，部分数据来源于NoteExpress
为什么在涉及细胞的分级分离时，这是一重要的考虑？
Why is this an important consideration in studies involving cell fractionation?
分离器的分级分离效率是循环流化床锅炉性能的主要影响因素之一。
The fractional collection efficiency of separator of CFB boiler is one of the main facts which control the performance of a circulating fluidized bed boiler.
研究了混合溶剂法分级分离棕榈酸果糖单酯和棕榈酸果糖双酯的工艺。
The fractionation separation of fructose monopalmitate and fructose dipalmitate by extraction with mixed organic solvent were performed.
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