...后，带电表层使它们相互排斥，而蛋白 和水分子之间的作用也加强，使蛋白的溶解度提高的现象 分段盐析（fractional salting out）：各种蛋白亲水性和荷电性不同，可以调节中性盐浓度， 使混合蛋白溶液中蛋白分散沉淀析出。 2.
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本文用二步分段盐析法，制备了胎儿淋巴细胞抑制因子(FLIF)。
Fetal lymphocyte inhibitory factors (FLIF) are prepared by means of the successive two-step salting-out procedure using ammonium sulphate.
分段盐析的提纯倍数为（以粗酶提取液为参照）褐藻酸酶13.3 ，琼脂酶8.7和纤维素酶10.9。
The number of purification times of algalase was 13.3 (crude extraction liquid as control ), 8.7 for agaraseand 10.9 for cellulase.
用高速离心、分段盐析、亲和层析、超滤和阴离子交换层析法，从牛胎盘中分离纯化出一种肝细胞生长因子。
A kind of hepatocyte growth factor was purified from cow placenta through high speed centrifugation, salting out, affinity chromatography, ultrafiltration and anion exchange chromatography.
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