冻融法是指采用反复冷冻与融化时由于细胞中形成了冰晶及剩余液体中盐浓度的增高可以使细胞破裂。这种方法简单方便，但要注意那些对温度变化敏感的蛋白质不宜采用此法。
探讨了冻融法的作用机制。
The mechanism of freezing and thawing method was also discussed.
目的比较酸洗涤法和冻融法提取HL60细胞抗原多肽的质量。
Objective To compare the qualities of HL60 cell antigen peptides extracted with acid elution or freeze thaw method.
结论二次乳化法和冻融法结合能显著提高5-氟尿嘧啶脂质体的包封率。
Conclusion Double emulsification and freeze-thawing method can significantly increase the encapsulation efficiency 5-FU in liposomes.
应用推荐