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根据我们的经验，简要地介绍了冷冻断裂蚀刻复型的方法和原理。
On the basis of our own experience, the procedures and the principle of the Freeze-fracture-etching-replica technique are briefly described.
本实验利用冷冻断裂技术和扫描电镜观察了瘤细胞侵入器官后的微细结构的变化。
The present experiment applied the freeze cracking technique and scanning electron microscopy for further studies of ultrastructural changes of cancer cells after they invaded into the organ.
结论红细胞冷冻断裂电镜技术可作为诊断dmd的辅助检查手段，也可作为检测d MD基因携带者的一种方法。
Conclusion The erythrocyte freeze-fracture electron microscopic technology may serve as a method for accessory examination of diagnosing DMD patients and a method for detecting DMD carriers.
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