其中，绝大多数藻类是采用两步冰冻法保存。
Most of them were preserved by the two_step freezing method.
应用冰冻法和透析法研究了柞蚕和家蚕丝胶的提取方法。
The different distilling methods freeze- method and dialyze- method of tussah and silkworm sericin were studied.
水稻组织培养物超低温保存的效果常受到材料特性、冰冻保护剂、降温冰冻法、后处理及恢复培养等因素的影响。
Cryopreservation of tissue cultures of rice is usually influenced by some factors, such as material properties, cryoprotectant, freezing procedure, treatment after freezing and renewing culture.
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