介绍了应用激光扫描共聚焦显微镜，结合免疫荧光技术研究卵母细胞和早期胚胎细胞骨架的方法。
This study systematically introduced the method of cytoskeleton detection in mouse oocytes and preimplantation embryos by confocal laser scanning microscopy and immunofluorescent staining technique.
方法采用细胞免疫荧光技术通过免疫荧光显微镜和激光共聚焦显微镜观察HPK1是否在大鼠脑细胞中存在；
Methods Immunofluorescence technique was used to investigate whether there was HPK1 in hippocampus neuron under the fluorescence microscope and the confocal microscopy.
方法免疫荧光组织化学双重染色，染色结果在激光共聚焦扫描显微镜下观察。
METHODS Immunofluorescence histochemical double staining technique was used and the staining results were observed with confocal laser scanning microscope.
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