目的探讨在不同的免疫学检测方法中，与单链抗体相连的亲和标记肽对单链抗体与抗原结合活性的影响。
Objective to investigate the affects of affinity tag on binding activity of single chain variable fragment (ScFv) to antigen using different immunological assays.
方法应用上皮性抗体和间叶性抗体标记喉梭型细胞癌细胞成分，观察其免疫学特性。
Methods Use epidemic antibody and mesenchyme antibody to mark the cell of spindle cell carcinoma, to observe their immunological character.
结论32p标记供体白细胞预处理受体免疫学指标未见显著改变，提示该方法是安全可靠的，为抗角膜移植免疫排斥反应提供了新的手段。
Conclusion The immunological indexes were not significantly changed after pretreatment of receptor by 32p labeled white blood cell, suggesting that such treatment is safe for cornea transplantation.
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