使用无菌技术。

Use aseptic technique.

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如果膜过滤技术是不适合的，则使用在供试产品无菌检查法项下的培养基直接接种法。

If the membrane filtration technique is unsuitable, use the Direct Inoculation of the Culture Medium method under Test for Sterility of the Product to be Examined.

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研究中使用了通过特殊饲养技术获得的无菌小鼠。这种小鼠可以接受人类的肠道菌类在其肠道中繁殖。

Researchers started with mice that were specially bred to be germ-free - with no gut microbiota of their own - and to be able to nurture human gut microbiota instead.

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