转录通常发生在生物体内，如果我们将含有RNA转录酶、NTP等条件，在体外无细胞系统中，用DNA作为模板，模仿体内转录过程生成RNA，这样的技术则能够控制转录的基因、转录的过程和转录后RNA的用途，该技术被称为体外转录。
A series of deleted sequences were obtained by PCR and were subcloned into expression vectors. Processing of the P1-polyprotein was verified by in vitro transcription/translation using T7 RNA polymerase transient expression system.
使用T7 RNA聚合酶体外瞬时表达系统,在细胞中表达T7 RNA聚合酶以启动载体的体外转录和翻译。
参考来源 - 口蹄疫病毒空衣壳蛋白的表达加工及其免疫原性研究·2,447,543篇论文数据，部分数据来源于NoteExpress
目的检测犬贾第虫病毒介导的锤头状核酶对犬贾第虫滋养体核仁功能性蛋白krr1基因体外转录体切割效率。
Objective to detect the cleavage activity of Giardia Canis virus (GCV) transfer vector-mediated hammerhead ribozyme for KRR1 in vitro transcript.
实验选用3-磷酸甘油醛脱氢酶基因（GAPDH）作为内参照，体外转录的RNA和阳性质粒作为两种标准品。
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene was served as the internal reference, and RNA transcribed in vitro and positive plasmid were served as the standards in this experiment.
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