通过比较发现,管渗法制备的染色体比整体低渗法的形态好。
The comparison of the two method declared that the morphea of the chromosome using cell low-osmosis was better than that of using embryo low-osmosis method.
方法采用急性分离的小鼠心室肌细胞,低渗溶液激活容积敏感性氯通道电流,膜片钳全细胞记录法记录电流。
Methods Isolated mouse cardiac ventricular myocytes were subjected to whole-cell patch-clamp to record the hypotonicity activated chloride channel currents.
方法用淋巴细胞分层法和低渗溶血法提取PBMC。
Methods Peripheral blood mononuclear cells(PBMC) were collected by using lymphocyte Ficoll and the low-osmotic hemolysis method.
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