表达产物用亲和层析一步法进行纯化，然后用凝胶阻滞试验观察重组蛋白结合质粒的能力。
The expressed protein was purified by one-step affinity chromatography, and its ability of binding plasmid was investigated via gel retardation experiments.
用谷胱甘肽琼脂糖凝胶4b亲和介质从菌体裂解液中纯化了GST - MBD4融合蛋白。
The GST-MBD4 fusion protein was purified from cell lysates using glutathione Sepharose 4b affinity chromatography.
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