方法 经密度梯度离心法分离出健康成年人外周血中的单个核细胞(mononuclear cells,MNCs),在体外诱导分化后,用FITC标记的荆豆凝集素Ⅰ(Dil-acLDL)和Dil标记的乙酰化低密度脂蛋...
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方法 经密度梯度离心法分离出健康成年人外周血中的单个核细胞(mononuclear cells,MNCs),在体外诱导分化后,用FITC标记的荆豆凝集素Ⅰ(Dil-acLDL)和Dil标记的乙酰化低密度脂蛋...
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外周血中的单个核细胞 peripheral blood mononuclear cell ; PBMCS
目的分析不同刺激下外周血单个核细胞(PBMC)中THANK基因的表达，并克隆全长的人THANK基因。
Aim to analyze THANK gene expression in peripheral blood mononuclear cells (PBMC) stimulated with different stimulators and to clone whole-length human THANK gene.
输注前从产品袋中留取标本检查有核细胞计数、单个核细胞比例、CD34+细胞计数和细胞的成活率（台盼蓝拒染率）。
The nucleated cell count, percentage of mononuclear cell , number of CD34+ cell and percentage of viable cell (trypan blue excluding rate) in the component were detected.
方法：用碱性磷酸酶抗碱性磷酸酶法检测再障患者骨髓中T淋巴细胞表型的变化，并用半固体培养法检测再障患者骨髓及去T细胞后骨髓单个核细胞CFU-GM集落形成情况。
Methods: Phenotype of T lymphocytes in bone marrow and CFU GM yields of bone marrow mononuclear cells with or without T cells were assayed with APAAP and semi solid culture method, respectively.
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