在锤头型核酶下游增加一段核酶作用的靶序列,使之成为自切割的核酶。
A self cleaving ribozyme was comprised of a hammerhead ribozyme and its target sequence located the downstream of the hammerhead ribozyme.
在FMDV的核苷酸序列相对保守区设计上下游引物FM1/FM4,扩增病毒的基因片段。
Then capture FMDV from clinical material with sterilized Eppendrof tubes of coating type-specific IgG, Amplify the conserved viral sequences by common primers (FM1/FM4).
应用谱分析对长江下游和西江下游感潮河段水文序列进行了周期识别,获得了可信的隐含周期。
The cryptic period of the hydrologic time series of tidal reaches of the Yangtze River and Xijiang River are analyzed with the applying of spectral analysis and the result is reasonable.
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