Photorespiration (also known as the oxidative photosynthetic carbon cycle, or C2 photosynthesis) is a process in plant metabolism which attempts to reduce the consequences of a wasteful oxygenation reaction by the enzyme RuBisCO. The desired reaction is the addition of carbon dioxide to RuBP (carboxylation), a key step in the Calvin–Benson cycle, however approximately 25% of reactions by RuBisCO instead add oxygen to RuBP (oxygenation), producing a product that cannot be used within the Calvin–Benson cycle. This process reduces efficiency of photosynthesis, potentially reducing photosynthetic output by 25% in C3 plants. Photorespiration involves a complex network of enzyme reactions that exchange metabolites between chloroplasts, leaf peroxisomes and mitochondria.The oxygenation reaction of RuBisCO is a wasteful process because 3-Phosphoglycerate is created at a reduced rate and higher metabolic cost compared with RuBP carboxylase activity. While photorespiratory carbon cycling results in the formation of G3P eventually, there is still a net loss of carbon (around 25% of carbon fixed by photosynthesis is re-released as CO2) and nitrogen, as ammonia. The ammonia must be detoxified at a substantial cost to the cell. Photorespiration also incurs a direct cost of one ATP and one NAD(P)H.While it is common to refer to the entire process as photorespiration, technically the term refers only to the metabolic network which acts to rescue the products of the oxygenation reaction (phosphoglycolate).