• 1. nested PCR-SSP: 序列特异引物
• 2. nested PCR-SSP typing: 巢式PCR
• 3. Nested SSP-PCR: 巢式序列特异引物扩增

• 1.In this study, a nested PCR SSP and a direct amplification PCR SSP protocols for HLA DR genotyping were developed and were used in the selection of matched donor for sibling BMT.
  该研究通过设计合成hla基因序列特异性引物，建立了HLA - DR基因分型的套式扩增和直接扩增pcr - SSP技术，并在骨髓移植配型中进行了应用。
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