而载体整合方式、整合拷贝数、5’非翻译区的改造和甲醇利用表型等对表达量无规律性影响。
The data also showed that the integrate site, copy number of expression vectors, 5' untranslated region and methanol utilization type had no obvious influence on yield of HSA.
目的：利用已有质粒构建带GF P报告基因的启动子鉴定质粒，并用此质粒鉴定丙型肝炎病毒5非翻译区(HCV 5'-UTR)启动基因表达的活性。
Objective; To construct a promoter identifying plasmid with GFP as reporter gene, and then identify the promoter activity of HCV 5 '- UTR with this construct.
目的：利用已有质粒构建带GF P报告基因的启动子鉴定质粒，并用此质粒鉴定丙型肝炎病毒5非翻译区(HCV 5'-UTR)启动基因表达的活性。
Objective; To construct a promoter identifying plasmid with GFP as reporter gene, and then identify the promoter activity of HCV 5 '- UTR with this construct.
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