为便于从融合蛋白中解离出目的多肽,这个载体中含有特异的蛋白酶裂解位点。
This vector also contains thrombin protease cleavage site to facilitate proteolysis of the bacterial fusion proteins.
对其氨基酸序列进行分析,蛋白酶裂解位点序列除LH株为GKRKKR外,其余三株为RRRKKR,为高致病性禽流感病毒的特征序列;
The proteolytic cleavage sites were all hexylbasic amino acids RRRKKR, with exception of LH (GKRKKR), which were the characteristic sequence of the highly pathogenic strains;
当HCA抑制柠檬酸裂解酶,脂肪生成过程停止。
When HCA inhibits citrate lyase, the fat-making process is halted.
蛇毒类凝血酶属于纤维蛋白原裂解酶,是一种丝氨酸蛋白酶。
Snake venom thrombin-like enzymes belong to the fibrinogenolytic enzyme family and are serine proteases.
向细胞裂解液中加入蛋白酶抑制剂后可以保护细胞提取物中的靶蛋白。
The addition of protease inhibitors to the cell lysis buffer AIDS in the preservation of target proteins in the cell extract.
在果胶酶中,原果胶酶、聚半乳糖醛酸酶、裂解酶和果胶酯酶得到了广泛深入的研究。
Extensive study of some pectinase such as protopectinases, polygalacturonases, lyases and pectin esterases has been done up to the present.
褐藻胶裂解酶能裂解褐藻胶制备寡糖,是一种重要的工具酶。
Alginate lyase can degrade alginates into oligosaccharides, and it's a kind of important tool enzyme.
近年来又发现了果胶酸裂解酶还可用于植物病毒的纯化、纸浆漂白和以纺织品的生物精炼等。
It was also found recently that the pectate lyase could be used for the purification of plant virus, bleaching of paper pulp and the bio-refining of textiles etc.
胰蛋白酶水解位点分析表明C肽无裂解位点,而A肽和B肽则分别具有3个和4个潜在水解位点。
No putative trypsin hydrolysis site was found in C peptide, however, 4 and 3 potential sites were found in A and B peptides respectively.
向细胞裂解液中加入磷酸酶抑制剂后可以保护细胞裂解时磷酸化残基。
The addition of phosphatase inhibitors to the cell lysis buffer AIDS in the preservation of phosphorylated residues at the time of cell disruption.
依托泊苷能稳定酶催化性循环中的一种共价的拓扑异构酶ii裂解的DNA中间复合物,导致基因组不稳定性和细胞死亡(2,3)。
Etoposide stabilizes a covalent topoisomerase II-cleaved DNA intermediate complex in the catalytic cycle of the enzyme, leading to genomic instability and cell death (2, 3).
以彩叶植物紫叶李为试验材料,研究了水分胁迫对紫叶李叶片中色素含量和苯丙氨酸裂解酶(PAL)活性的影响。
Effects of water stress on pigment content and PAL activity of purple leaf plum, a color-leafed plant, were studied.
当细胞被裂解提取全细胞提取物时,会发生正常的细胞调节缺失,并且细胞提取物中的内源性蛋白酶可以在失控的情况下使蛋白发生降解。
A loss of normal cellular control occurs during cell lysis, and endogenous proteases within the cell extract are free to degrade proteins in an uncontrolled manner.
在这个实施方案中,底物为蛋白水解酶提供裂解目标。
In this embodiment, the substrate provides a cleavage target for a proteolytic enzyme.
噬菌体裂解酶是双链DNA噬菌体所特有的细胞壁水解酶。
Bacteriophage lysins are lytic agents used by double-stranded DNA(dsDNA) phages to coordinate bacterial cell wall lysis.
腺苷酸琥珀酸裂解酶(ADSL)是催化嘌呤核苷酸的从头合成与嘌呤核苷酸循环的双功能酶。
Adenylosuccinate lyase (ADSL) is a bifunctional enzyme catalyzing denovo purine synthesis and purine nucleotide recycling.
本文建立了蛋白酶体裂解内源性抗原蛋白的QSAR模型来研究蛋白酶体对抗原蛋白裂解的特异性,并采用PLS方法求解模型。
The QSAR model of proteasomal cleaving antigen protein is built in order to study the specificity of the proteasome cleaving antigen protein, and PLS method is used to solve the model.
该载体是含有异 分支酸合成酶基因和异分支酸-丙酮酸裂解酶基因的植物表达载体。
The carrier is the plant expressing carrier containing the separating offset acid composing enzyme gene and the separating offset acid-the pyruvic splitting dividing enzyme gene.
建立了用反相高效液相色谱测定青霉素G酶促裂解产物的方法。
RP-HPLC method for analyzing enzymatic hydrolysate of penicillin G was established.
吲哚胺2,3双加氧酶(IDO)是一种免疫调节酶,可催化色氨酸分子中吲哚环氧化裂解,从而沿犬尿酸途径分解代谢的限速酶。
The immunoregulatory enzyme indoleamine 2,3-dioxygenase (IDO) catalyzes the oxidative cleavage of the indole ring of tryptophan.
本文对海藻酸盐裂解酶的生物特性、研究方法及其生物学功能进行了介绍。
This review catalogs the major characteristics of these lyases, the methods for analyzing these enzymes, as well as their biological roles.
在免疫激活剂昆布多糖存在下,分别测得小菜蛾幼虫血细胞碎片、血细胞裂解液和血浆的酚氧化酶活性为26.80U, 16.68 U和2.53 U。
When laminarin as an immune activator was present, phenoloxidase (PO) activities in cell debris, hemocyte lysate solution and plasma were measured as 26.80, 16.68, and 2.53 u, respectively.
测定了降解途径中相关酶的活性,表明对氯苯胺经过苯胺双加氧酶初始氧化和羟基化后,芳环的裂解是由邻苯二酚2 ,3双加氧酶催化。
Enzymatic analysis show that initial reactions ofp-chloroaniline degradation by Diaphorobacter sp. PCA039 are catalyzed by aniline dioxygenase and chlorocatechol 2,3-dioxygenase.
指数生长期的SEMcells (106cells/ml)用加或不加磷酸酶抑制剂的裂解液进行裂解。
SEM cells in exponential growth (106 cells/ml) were lysed with or without the addition of phosphatase inhibitor to the lysis buffer (phospho or nonphospho lysate).
指数生长期的SEMcells (106cells/ml)用加或不加磷酸酶抑制剂的裂解液进行裂解。
SEM cells in exponential growth (106 cells/ml) were lysed with or without the addition of phosphatase inhibitor to the lysis buffer (phospho or nonphospho lysate).
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